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interleukin 1β  (R&D Systems)


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    Structured Review

    R&D Systems interleukin 1β
    Interleukin 1β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+rat+interleukin+1%CE%B2/Recombinant+Rat+IL-1+beta%2FIL-1F2+Protein/pmc11561464__mmc2-371-12-20
    Average 93 stars, based on 47 article reviews
    interleukin 1β - by Bioz Stars, 2026-10
    93/100 stars

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    Recombinant:

    Article Title: HIV-1 protein Tat produces biphasic changes in NMDA-evoked increases in intracellular Ca 2+ concentration via activation of Src kinase and nitric oxide signaling pathways
    Article Snippet: .. Materials were obtained from the following sources: HIV-1 Tat (Clade B, full length recombinant) was from the NIH AIDS Research and Reference Reagent Program (HIV-1 Tat protein (full length, Clade B) from John Brady and DAIDS, NIAID and from Prospec Tany TechnoGene Ltd. (Rehovot, Israel); recombinant rat low-density lipoprotein receptor-related protein associated protein 1 (RAP) was from Fitzgerald Industries International (Concord, MA); Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum, horse serum, fura-2-acetomethyl ester (Fura-2-AM), and glycine were from Invitrogen (Carlsbad, CA); NMDA, NGNitro-L-Arginine Methyl Ester (L-NAME), and 1H-(1,2,4)oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) were from Sigma-Aldrich (St. Louis, MO); 4-amino-5-(4-chloro- phenyl)-7-( t- butyl)pyrazolo[3,4- d ]pyrimidine (PP2) and 4-amino-7-phenylpyrazol[3,4-d]pyrimidine (PP3), 8R,9S,11S)-(-)-9-methoxy-carbamyl-8-methyl-2,3,9,10-tetrahydro-8,11-epoxy-1H,8H,11H-2,7b,11a-trizadibenzo-(a,g)-cycloocta-(c,d,e)-trinden-1-one (KT5823), and (1 R *,2 S *)- erythro -2-(4-Benzylpiperidino)-1-(4-hydroxyphenyl)-1-propanol hemitartrate (ifenprodil) were from Tocris (Bristol, United Kingdom); 2-(2-amino-3-methyoxyphenyl)-4 H -1-benzopyran-4-one (PD98059) was from Cell Signaling Technology (Danvers, MA); recombinant rat interleukin-1β was from R&D Systems (Minneapolis, MN). ..

    Article Title: HIV-1 protein Tat produces biphasic changes in NMDA-evoked increases in intracellular Ca 2+ concentration via activation of Src kinase and nitric oxide signaling pathways
    Article Snippet: .. Drugs and Reagents Materials were obtained from the following sources: HIV-1 Tat (Clade B, full length recombinant) was from the NIH AIDS Research and Reference Reagent Program (HIV-1 Tat protein (full length, Clade B) from John Brady and DAIDS, NIAID and from Prospec Tany TechnoGene Ltd. (Rehovot, Israel); recombinant rat low-density lipoprotein receptor-related protein associated protein 1 (RAP) was from Fitzgerald Industries International (Concord, MA); Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum, horse serum, fura-2-acetomethyl ester (Fura-2-AM), and glycine were from Invitrogen (Carlsbad, CA); NMDA, NGNitro-L-Arginine Methyl Ester (L-NAME), and 1H-(1,2,4)oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) were from Sigma-Aldrich (St. Louis, MO); 4-amino-5-(4-chloro- phenyl)-7-( t- butyl)pyrazolo[3,4- d ]pyrimidine (PP2) and 4-amino-7-phenylpyrazol[3,4-d]pyrimidine (PP3), 8R,9S,11S)-(-)-9-methoxy-carbamyl-8-methyl-2,3,9,10-tetrahydro-8,11-epoxy-1H,8H,11H-2,7b,11a-trizadibenzo-(a,g)-cycloocta-(c,d,e)-trinden-1-one (KT5823), and (1 R *,2 S *)- erythro -2-(4-Benzylpiperidino)-1-(4-hydroxyphenyl)-1-propanol hemitartrate (ifenprodil) were from Tocris (Bristol, United Kingdom); 2-(2-amino-3-methyoxyphenyl)-4 H -1-benzopyran-4-one (PD98059) was from Cell Signaling Technology (Danvers, MA); recombinant rat interleukin-1β was from R&D Systems (Minneapolis, MN). ..

    Article Title: The role of spinal interleukin-1β and astrocyte connexin 43 in the development of mirror-image pain in an inflammatory pain model.
    Article Snippet: Article history: Received 20 April 2016 Received in revised form 6 October 2016 Accepted 18 October 2016 Available online 20 October 2016 Although we have recently demonstrated that carrageenan-induced inflammation upregulates the expression of spinal interleukin (IL)-1β, which inhibits spinal astrocyte activation and results in the delayed development of Mirror-Image Pain (MIP), little is known regarding the mechanisms that underlie how spinal IL-1β inhibits the astrocyte activation.. In this study, we examined the effect of spinal IL-1β on astrocyte gap junctions (GJ) and the development of MIP.. Following unilateral carrageenan (CA) injection, mechanical allodynia (MA) was evaluated at various time points.

    Modification:

    Article Title: HIV-1 protein Tat produces biphasic changes in NMDA-evoked increases in intracellular Ca 2+ concentration via activation of Src kinase and nitric oxide signaling pathways
    Article Snippet: .. Materials were obtained from the following sources: HIV-1 Tat (Clade B, full length recombinant) was from the NIH AIDS Research and Reference Reagent Program (HIV-1 Tat protein (full length, Clade B) from John Brady and DAIDS, NIAID and from Prospec Tany TechnoGene Ltd. (Rehovot, Israel); recombinant rat low-density lipoprotein receptor-related protein associated protein 1 (RAP) was from Fitzgerald Industries International (Concord, MA); Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum, horse serum, fura-2-acetomethyl ester (Fura-2-AM), and glycine were from Invitrogen (Carlsbad, CA); NMDA, NGNitro-L-Arginine Methyl Ester (L-NAME), and 1H-(1,2,4)oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) were from Sigma-Aldrich (St. Louis, MO); 4-amino-5-(4-chloro- phenyl)-7-( t- butyl)pyrazolo[3,4- d ]pyrimidine (PP2) and 4-amino-7-phenylpyrazol[3,4-d]pyrimidine (PP3), 8R,9S,11S)-(-)-9-methoxy-carbamyl-8-methyl-2,3,9,10-tetrahydro-8,11-epoxy-1H,8H,11H-2,7b,11a-trizadibenzo-(a,g)-cycloocta-(c,d,e)-trinden-1-one (KT5823), and (1 R *,2 S *)- erythro -2-(4-Benzylpiperidino)-1-(4-hydroxyphenyl)-1-propanol hemitartrate (ifenprodil) were from Tocris (Bristol, United Kingdom); 2-(2-amino-3-methyoxyphenyl)-4 H -1-benzopyran-4-one (PD98059) was from Cell Signaling Technology (Danvers, MA); recombinant rat interleukin-1β was from R&D Systems (Minneapolis, MN). ..

    Article Title: HIV-1 protein Tat produces biphasic changes in NMDA-evoked increases in intracellular Ca 2+ concentration via activation of Src kinase and nitric oxide signaling pathways
    Article Snippet: .. Drugs and Reagents Materials were obtained from the following sources: HIV-1 Tat (Clade B, full length recombinant) was from the NIH AIDS Research and Reference Reagent Program (HIV-1 Tat protein (full length, Clade B) from John Brady and DAIDS, NIAID and from Prospec Tany TechnoGene Ltd. (Rehovot, Israel); recombinant rat low-density lipoprotein receptor-related protein associated protein 1 (RAP) was from Fitzgerald Industries International (Concord, MA); Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum, horse serum, fura-2-acetomethyl ester (Fura-2-AM), and glycine were from Invitrogen (Carlsbad, CA); NMDA, NGNitro-L-Arginine Methyl Ester (L-NAME), and 1H-(1,2,4)oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) were from Sigma-Aldrich (St. Louis, MO); 4-amino-5-(4-chloro- phenyl)-7-( t- butyl)pyrazolo[3,4- d ]pyrimidine (PP2) and 4-amino-7-phenylpyrazol[3,4-d]pyrimidine (PP3), 8R,9S,11S)-(-)-9-methoxy-carbamyl-8-methyl-2,3,9,10-tetrahydro-8,11-epoxy-1H,8H,11H-2,7b,11a-trizadibenzo-(a,g)-cycloocta-(c,d,e)-trinden-1-one (KT5823), and (1 R *,2 S *)- erythro -2-(4-Benzylpiperidino)-1-(4-hydroxyphenyl)-1-propanol hemitartrate (ifenprodil) were from Tocris (Bristol, United Kingdom); 2-(2-amino-3-methyoxyphenyl)-4 H -1-benzopyran-4-one (PD98059) was from Cell Signaling Technology (Danvers, MA); recombinant rat interleukin-1β was from R&D Systems (Minneapolis, MN). ..

    Blocking Assay:

    Article Title: The role of spinal interleukin-1β and astrocyte connexin 43 in the development of mirror-image pain in an inflammatory pain model.
    Article Snippet: Article history: Received 20 April 2016 Received in revised form 6 October 2016 Accepted 18 October 2016 Available online 20 October 2016 Although we have recently demonstrated that carrageenan-induced inflammation upregulates the expression of spinal interleukin (IL)-1β, which inhibits spinal astrocyte activation and results in the delayed development of Mirror-Image Pain (MIP), little is known regarding the mechanisms that underlie how spinal IL-1β inhibits the astrocyte activation.. In this study, we examined the effect of spinal IL-1β on astrocyte gap junctions (GJ) and the development of MIP.. Following unilateral carrageenan (CA) injection, mechanical allodynia (MA) was evaluated at various time points.



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